Plasmid stability assays.

<p>(A) Plasmids pBAV1K-T5-<i>gfp</i>, pGK12, pLZ12-T5-<i>gfp</i>, pQBAV3Cm-T5-<i>gfp</i> and pIMBB-T5-<i>gfp</i> were separately propagated in <i>E. coli</i> for 80 generations without antibiotic selection. Plasmid stability was determined by replica plating onto selective media and presented as a percentage of cells that retain antibiotic resistance. (B) Plasmids pBAV1K-T5-<i>gfp</i>, pGK12, pLZ12-T5-<i>gfp</i>, were propagated in <i>B. subtilis</i> for 80 generations without antibiotic selection. Plasmid stability was determined by the plasmid content comparison in the total DNA pools between different generations. Error bars represent standard error.