p53 knockdown in BAR-T cells.

<p>(A) Western blot demonstrating expression of p53 and p21 at baseline and after irradiation with 200 J/m² UV-B in controls, vector-only cells, the entire p53 RNAi-containing population and a p53 RNAi-containing clone with nearly complete p53 knockdown; β-actin served as a loading control. (B) Population doubling time in BAR-T cells containing p53 RNAi. (*, p<0.05 compared to vector control) (C) Cell-to-cell contact inhibition in BAR-T cells containing p53 RNAi. Cell numbers increase significantly in a time-dependent manner up to day 8 in vector-only cells (p<0.001) and in the p53-knockdown population (p<0.001), and up to day 10 in the clone (p<0.05).