H-Ras^G12Vexpression in BAR-T p53 RNAi knockdown cells.

<p>(A) Representative western blot demonstrating H-Ras expression as well as phosphorylation of MEK1/2 and ERK1/2 in a clone containing vector only, and in two H-Ras^G12V-infected, p53 knockdown clones (R1 and R2); β-tubulin served as a loading control. (B) Population doubling time in a clone containing vector only, and in two H-Ras^G12V-infected, p53 knockdown clones (R1 and R2). (*, p<0.05 compared to vector control) (C) BAR-T p53 RNAi cells expressing H-Ras^G12V demonstrate loss of cell-to-cell contact inhibition. In contrast to a clone containing vector only in which cell numbers level off after day 10, cell numbers in the two H-Ras^G12V-infected, p53 knockdown clones (R1 and R2) continued to increase significantly in a time-dependent manner (p<0.001 for clones R1 and R2).