Methylation analysis of hsa-mir-9-1 gene in primary tumors.

<p>Methylation density of hsa-mir-9-1 gene in endemic BL cases negative for <i>MYC</i> translocation (NT, Negative for translocation) and reactive lymph nodes (C1, C2) by bisulfite sequencing; Solid circles (•) and empty circles (○) indicate that CpG site is methylated or unmethylated, respectively. The BL cases negative for <i>MYC</i> translocation showed aberrant (cases 1–5) and partial (cases 6 and 7) methylation, in respect with the normal controls (C1 and C2). Cases NT1 and NT2 showed 21/23 CpG methylated, case NT3 22/23 CpGs methylated, cases NT3 and NT4 showed all of CpGs methylated (23/23), case NT5 showed 12/23 CpG methylated and case NT6 showed 9/23 CpG methylated. On the other hand, the normal controls (C1, C2) showed 2/23 and 1/23 CpGs methylated, respectively.