Anti-TGFβ1 antibody neutralizes endogenous TGFβ1 and potentiates the decidualization process.

<p>Cultured ESCs were decidualized <i>in vitro</i> in the prescence and absence of TGFβ1. To confirm the specificity of the TGFβ1 response anti-TGFβ1 antibody (1 µg/ml) or mouse IgG control were added, for 72 h. A: TGFβ1 inhibited expression of decidual PRL (p<0.05), addition of anti-TGFβ1 blocked this reduction. 1.0 = absolute value of ΔCT = 11.5. B: TGFβ1 reduced expression of IGFBP-1 mRNA (p<0.001), whilst anti-TGFβ1 antibody increased expression IGFBP-1 (p<0.001) above that of controls. 1.0 = absolute value of ΔCT = 12.3. Data are mean ± S.E.M; * p<0.05, *** p<0.001. n = 6 endometrial samples (in triplicate).