Influence of CD28 and NKG2D-mediated co-stimulation in cytotoxicity.

<p>Negatively selected CD3<b>⁺</b>CD8<b>⁺</b> T cells were further sorted based on their CD28 expression levels into CD28^Lo and CD28^Hi CD8<b>⁺</b> T cells. These cells were activated with plate-bound antibodies directed against CD3, CD28 and NKG2D in different combinations. Cell surface expression of CD107a was analyzed through flow cytometry and used as a measure of cytotoxicity. Three independent samples were used to obtain data and P values were calculated using paired t-test.