TNF regulates Notch-1 expression in vivo.

<p><b>A.</b> Muscle homogenates were prepared from 7–8 week old <i>mdx</i> mice that either contained or lacked IKKβ and western analysis was performed probing for Notch-1. The blot was stripped and reprobed for α-tubulin used as a loading control. <b>B.</b> Nude mice were implanted with vector control CHO cells or CHO cells expressing TNF. Once tumors were established, muscle injury was induced to tibialis anterior muscles with cardiotoxin injections. At indicated days post-toxin injections, muscles were harvested and homogenates prepared for western analysis probing for Notch-1 and α-tubulin as a loading control.