Induction of MIE gene expression by CDK inhibition does not require nuclear localization of pp71.

<p>(A) Undifferentiated NT2 cells, differentiated NT2 cells (treated with RA for 7 days) and mouse bone marrow (BM) cells were analyzed for Cyclin A2 expression by immunoblot analysis. GAPDH expression was analyzed to control for equal loading. (B) Undifferentiated and differentiated NT2 cells were infected with HCMV-TB40/e (MOI = 5). Where indicated, SU9516 at 10 µM final concentration was added together with the virus. At 6 hpi cells were analyzed for subcellular localization of pp71 (green) by immunofluorescence microscopy. Nuclei were stained with DAPI fluorochrome (blue). Non-infected cells (right panel) were analyzed to control for background staining. Scale bars (red) = 10 µm.