Effects of <i>in vitro</i> B₁R activation on superoxide anion production in the aorta of glucose-fed rats pre-treated or not with oxidative enzyme inhibitors.

<p>Basal and stimulated production of superoxide anion in the presence of the B₁R agonist Sar[D-Phe⁸]des-Arg⁹-BK (20 µM) were measured in the presence of (A) Allopurinol (xanthine oxidase inhibitor), (B) L-NAME (inhibitor of all NOS isoforms) and (C) Apocynin (NADPH oxidase inhibitor) at the indicated concentrations (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012622#s2" target="_blank">methods</a>). Data are mean ± s.e.m of values obtained from (n) rats. Statistical comparison with control (*), glucose (+) or Sar[D-Phe⁸]des-Arg⁹-BK (†) is indicated by * ⁺ P< 0.05, ** ^{++ ††}P< 0.01.