Phagocytosis of ΔLdMT and ΔLdRos3 parasites by THP-1-derived macrophages is unchanged.

<p>CellTrackerTM Green-labelled parasites were added (10∶1) to THP-1-derived macrophages prelabelled with CellTrackerTM Dil (red) and co-incubated for 16 h at 37°C. (A) Micrograph of double-fluorescence labelling of THP-1-derived macrophages phagocytosing <i>Leishmaina</i> parasites. Bar, 10 µm. (B) Percentage of phagocytosing THP-1-derived macrophages was determined by flow cytometry. Values are means ± SD of three independent experiments expressed as percentage of control (number of phagocytosed wild-type parasites by TPH-1-derived macrophages). The population of phagocytosing THP-1-derived macrophages ranged from 30 to 70% in control cultures.