Lymphocyte co-culture facilitates MDCK tight junction assembly.

<p>(a) Representative pictures of ZO-1 relocation to cell–cell junctions in MDCK cells cultured alone and co-cultured with lymphocytes at various time points (0h, 0.5h, 1h, 1.5h, 2h, 2.5h, 3h) after calcium switch. (Scale bar: 30 µm). (b) Quantification of ZO-1 relocation to cell–cell junctions (length of ZO-1/cell (µm)) in MDCK cells cultured alone and co-cultured with lymphocytes at various time points (0h, 0.5h, 1h, 1.5h, 2h, 2.5h, 3h) after calcium switch. The asterisks denote significant differences detected in the presence vs. the absence of lymphocytes by Student's <i>t</i> test (*, p<0.05; **, p<0.01).