Changes in LC numbers and morphology in epidermal sheets.

<p>Human epidermal sheets were isolated from untreated cultured skin and cultured skin treated by ID injection of PBS (control) or H1 VLPs. (<b>A</b>) Epidermal sheets were stained for CD207 and positively stained cells were counted. Data presented as the percentage of CD207+ve cells (LCs) relative to cell numbers in untreated skin at each timepoint. Skin treated by ID delivery of PBS (grey bars); Skin treated by ID delivery of H1 VLPs (white bars); data presented as mean ± SD (n = 4), significance was determined relative to untreated skin at each timepoint (***p<0.001). (<b>B</b>) Representative images of LCs (CD207+ve cells) taken from each time point and treatment (bar = 20µm). (<b>C</b>) LCs displaying a “hyper-dendritic “cell morphology. (<b>D</b>) LCs displaying “rounded” cell morphology. (<b>E</b>) Both hyper-dendritic and rounded LCs observed in the same optical plane of the epidermis (bar = 10µm all cases).