Mass spectra of the Endopep-MS BoNT/A reactions with /A1 (1A), /A2 (1B), /A3 (1C), /A4 (1D), or no toxin (1E) extracted using the 4E17.1 antibody-coated beads.

Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are prot...

Mass spectra for crosslinks identified in protein multisubunit complexes.

Christopher A. G. Söderberg (319134)
Wietske Lambert (319136)
Sven Kjellström (319138)
Alena Wiegandt (319140)
Ragna Peterson Wulff (319141)
Cecilia Månsson (319142)
Gudrun Rutsdottir (319143)
Cecilia Emanuelsson (319144)

February 2013

The Clp-protease multisubunit protein complex was crosslinked and the tryptic digest was separate...

Improved identification of endogenous peptides from murine nervous tissue by multiplexed peptide extraction methods and multiplexed mass spectrometric analysis.

Altelaar, AF
Mohammed, S
Brans, MA
Adan, RA
Heck, AJ

January 2009

In recent years, mass spectrometry (MS) based techniques have made their entrance in the analysis of...

Mass spectra of the Endopep-MS BoNT/E reactions with /E1 (3A), /E2 (3B), /E3 (3C), /E4 (3D), or no toxin (3E) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/E are m/z 1136.6 and 2923.6 and the peptide ...

Mass spectra of the Endopep-MS BoNT/B reactions with /B1 (2A), /B2 (2B), /B3 (2C), /B4 (2D), /B5 (2E), or no toxin (2F) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/B are m/z 1759.9 and 2283.4 and the peptide ...

Mass spectra of the Endopep-MS BoNT/F reactions with /F1 (4A), /F6 (4B), /F2 (4C), or no toxin (4D) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/F are m/z 1345.2 and 3167.8 and the peptide ...

Mass spectra of the Endopep-MS BoNT/D reactions with no antibody extraction (5A), extraction with monoclonal antibody 4E17.1 (5B), or extraction with monoclonal antibody CR2 (5C).

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/D are m/z 1217.7 and 3296.9 and the peptide ...

Mass spectra from the reaction of peptide substrates with 4E17.1 coated beads used to extract A2b (7A), Ba4 (7B), Af (7C), and Bf (7D).

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Peaks at m/z 1197.8 and 1699.9 indicate BoNT/A, peaks at m/z 1759.9 and 2283.4 indi...

Peak area ratios of the peptide cleavage product over the internal standard peptide obtained from the Endopep-MS reaction of BoNT/A with its peptide substrate in the presence of the antibody panel.

Suzanne R. Kalb (362450)
Jianlong Lou (362452)
Consuelo Garcia-Rodriguez (362451)
Isin N. Geren (373081)
Theresa J. Smith (82184)
Hercules Moura (373082)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Four different forms of BoNT A were used, and they include /A1, A2, A3, and A1 light chain.</p

Peak area ratios of the peptide cleavage product over the internal standard peptide obtained from the Endopep-MS reaction of BoNT A after its extraction by the antibody panel.

Suzanne R. Kalb (362450)
Jianlong Lou (362452)
Consuelo Garcia-Rodriguez (362451)
Isin N. Geren (373081)
Theresa J. Smith (82184)
Hercules Moura (373082)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Three different forms of BoNT A were used, and they include A1, A2, and A3.</p

Comparison of the catalytic activity of BoNT/A1 and BoNT/A8.

Skadi Kull (690586)
K. Melanie Schulz (690587)
Jasmin Weisemann née Strotmeier (690588)
Sebastian Kirchner (170385)
Tanja Schreiber (690589)
Alexander Bollenbach (690590)
P. Wojtek Dabrowski (690591)
Andreas Nitsche (12987)
Suzanne R. Kalb (362450)
Martin B. Dorner (170395)
John R. Barr (71845)
Andreas Rummel (469246)
Brigitte G. Dorner (170398)

February 2015

(A) Quantitative mass spectrometric endopeptidase assay of native full-length BoNT/A1 and BoNT/A8...

mAb inhibition of BoNT/A LC endopeptidase activity.

Yongfeng Fan (783637)
Isin N. Geren (373081)
Jianbo Dong (783638)
Jianlong Lou (362452)
Weihua Wen (783639)
Fraser Conrad (783640)
Theresa J. Smith (82184)
Leonard A. Smith (82190)
Mengfei Ho (280179)
Melissa Pires-Alves (783641)
Brenda A. Wilson (237558)
James D. Marks (82191)

August 2015

A. SDS-PAGE-based substrate cleavage assay: BoNT/A LC (25 nM) and 20 molar excess of mAb w...

Fragmentation spectrum of a peptide with accurate measured mass of 1209.7315 Da (M), doubly-charged precursor (m/z 605.3694).

Vladimir Gorshkov (1359993)
Wolfgang Blenau (120829)
Gudrun Koeniger (2211064)
Andreas Römpp (489928)
Andreas Vilcinskas (68058)
Bernhard Spengler (1235118)

May 2015

The sequence under the spectrum shows observed cleavages for complete peptide and for b10-ion. In...

Mass spectrometric approaches for elucidation of antigen antibody recognition structures in molecular immunology

Stefanescu, Raluca
Iacob, Roxana Elena
Damoc, Eugen
Marquardt, Andreas
Amstalden, Erika
Manea, Marilena
Perdivara, Irina
Maftei, Madalina
Paraschiv, Gabriela
Przybylski, Michael

January 2007

Mass spectrometric approaches have recently gained increasing access to molecular immunology and sev...

Preparation of affinity surfaces for mass spectrometry

Kalaninová, Zuzana

January 2021

One of the most potent toxic substances of biological origin known are botulinum toxins - nerve toxi...

Toxins (Basel)

Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are prot...

Mass spectra for crosslinks identified in protein multisubunit complexes.

Christopher A. G. Söderberg (319134)
Wietske Lambert (319136)
Sven Kjellström (319138)
Alena Wiegandt (319140)
Ragna Peterson Wulff (319141)
Cecilia Månsson (319142)
Gudrun Rutsdottir (319143)
Cecilia Emanuelsson (319144)

February 2013

The Clp-protease multisubunit protein complex was crosslinked and the tryptic digest was separate...

Improved identification of endogenous peptides from murine nervous tissue by multiplexed peptide extraction methods and multiplexed mass spectrometric analysis.

Altelaar, AF
Mohammed, S
Brans, MA
Adan, RA
Heck, AJ

January 2009

In recent years, mass spectrometry (MS) based techniques have made their entrance in the analysis of...

Mass spectra of the Endopep-MS BoNT/E reactions with /E1 (3A), /E2 (3B), /E3 (3C), /E4 (3D), or no toxin (3E) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/E are m/z 1136.6 and 2923.6 and the peptide ...

Mass spectra of the Endopep-MS BoNT/B reactions with /B1 (2A), /B2 (2B), /B3 (2C), /B4 (2D), /B5 (2E), or no toxin (2F) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/B are m/z 1759.9 and 2283.4 and the peptide ...

Mass spectra of the Endopep-MS BoNT/F reactions with /F1 (4A), /F6 (4B), /F2 (4C), or no toxin (4D) extracted using the 4E17.1 antibody-coated beads.

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/F are m/z 1345.2 and 3167.8 and the peptide ...

Mass spectra of the Endopep-MS BoNT/D reactions with no antibody extraction (5A), extraction with monoclonal antibody 4E17.1 (5B), or extraction with monoclonal antibody CR2 (5C).

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

The peptide cleavage products indicating BoNT/D are m/z 1217.7 and 3296.9 and the peptide ...

Mass spectra from the reaction of peptide substrates with 4E17.1 coated beads used to extract A2b (7A), Ba4 (7B), Af (7C), and Bf (7D).

Suzanne R. Kalb (362450)
Consuelo Garcia-Rodriguez (362451)
Jianlong Lou (362452)
Jakub Baudys (362453)
Theresa J. Smith (82184)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Peaks at m/z 1197.8 and 1699.9 indicate BoNT/A, peaks at m/z 1759.9 and 2283.4 indi...

Peak area ratios of the peptide cleavage product over the internal standard peptide obtained from the Endopep-MS reaction of BoNT/A with its peptide substrate in the presence of the antibody panel.

Suzanne R. Kalb (362450)
Jianlong Lou (362452)
Consuelo Garcia-Rodriguez (362451)
Isin N. Geren (373081)
Theresa J. Smith (82184)
Hercules Moura (373082)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Four different forms of BoNT A were used, and they include /A1, A2, A3, and A1 light chain.</p

Peak area ratios of the peptide cleavage product over the internal standard peptide obtained from the Endopep-MS reaction of BoNT A after its extraction by the antibody panel.

Suzanne R. Kalb (362450)
Jianlong Lou (362452)
Consuelo Garcia-Rodriguez (362451)
Isin N. Geren (373081)
Theresa J. Smith (82184)
Hercules Moura (373082)
James D. Marks (82191)
Leonard A. Smith (82190)
James L. Pirkle (17765)
John R. Barr (71845)

February 2013

Three different forms of BoNT A were used, and they include A1, A2, and A3.</p

Comparison of the catalytic activity of BoNT/A1 and BoNT/A8.

Skadi Kull (690586)
K. Melanie Schulz (690587)
Jasmin Weisemann née Strotmeier (690588)
Sebastian Kirchner (170385)
Tanja Schreiber (690589)
Alexander Bollenbach (690590)
P. Wojtek Dabrowski (690591)
Andreas Nitsche (12987)
Suzanne R. Kalb (362450)
Martin B. Dorner (170395)
John R. Barr (71845)
Andreas Rummel (469246)
Brigitte G. Dorner (170398)

February 2015

(A) Quantitative mass spectrometric endopeptidase assay of native full-length BoNT/A1 and BoNT/A8...

mAb inhibition of BoNT/A LC endopeptidase activity.

Yongfeng Fan (783637)
Isin N. Geren (373081)
Jianbo Dong (783638)
Jianlong Lou (362452)
Weihua Wen (783639)
Fraser Conrad (783640)
Theresa J. Smith (82184)
Leonard A. Smith (82190)
Mengfei Ho (280179)
Melissa Pires-Alves (783641)
Brenda A. Wilson (237558)
James D. Marks (82191)

August 2015

A. SDS-PAGE-based substrate cleavage assay: BoNT/A LC (25 nM) and 20 molar excess of mAb w...

Fragmentation spectrum of a peptide with accurate measured mass of 1209.7315 Da (M), doubly-charged precursor (m/z 605.3694).

Vladimir Gorshkov (1359993)
Wolfgang Blenau (120829)
Gudrun Koeniger (2211064)
Andreas Römpp (489928)
Andreas Vilcinskas (68058)
Bernhard Spengler (1235118)

May 2015

The sequence under the spectrum shows observed cleavages for complete peptide and for b10-ion. In...

Mass spectrometric approaches for elucidation of antigen antibody recognition structures in molecular immunology

Stefanescu, Raluca
Iacob, Roxana Elena
Damoc, Eugen
Marquardt, Andreas
Amstalden, Erika
Manea, Marilena
Perdivara, Irina
Maftei, Madalina
Paraschiv, Gabriela
Przybylski, Michael

January 2007

Mass spectrometric approaches have recently gained increasing access to molecular immunology and sev...

Preparation of affinity surfaces for mass spectrometry

Kalaninová, Zuzana

January 2021

One of the most potent toxic substances of biological origin known are botulinum toxins - nerve toxi...

Toxins (Basel)

Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are prot...

Mass spectra for crosslinks identified in protein multisubunit complexes.

Christopher A. G. Söderberg (319134)
Wietske Lambert (319136)
Sven Kjellström (319138)
Alena Wiegandt (319140)
Ragna Peterson Wulff (319141)
Cecilia Månsson (319142)
Gudrun Rutsdottir (319143)
Cecilia Emanuelsson (319144)

February 2013

The Clp-protease multisubunit protein complex was crosslinked and the tryptic digest was separate...

Improved identification of endogenous peptides from murine nervous tissue by multiplexed peptide extraction methods and multiplexed mass spectrometric analysis.

Altelaar, AF
Mohammed, S
Brans, MA
Adan, RA
Heck, AJ

January 2009

In recent years, mass spectrometry (MS) based techniques have made their entrance in the analysis of...

Mass spectra of the Endopep-MS BoNT/A reactions with /A1 (1A), /A2 (1B), /A3 (1C), /A4 (1D), or no toxin (1E) extracted using the 4E17.1 antibody-coated beads.

Abstract

Extracted data

Mass spectra of the Endopep-MS BoNT/A reactions with /A1 (1A), /A2 (1B), /A3 (1C), /A4 (1D), or no toxin (1E) extracted using the 4E17.1 antibody-coated beads.

Abstract

Extracted data

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