hCNS-SCns mostly differentiate into oligodendrocytes and neurons, and few astrocytes.

<p>(<b>A–E</b>) Several human nuclei positive cells, green (<b>C</b>), expressed Olig2 marker revealing immature oligodendrocytes, red (<b>B</b>), DAPI counterstain, blue (<b>A</b>). Arrows indicate a double-labeled cell. Merged confocal image, showing hCNS-SCns expression of Olig2 indicating differentiation to oligodendrocytes (<b>D</b>). Orthogonal view of confocal image showing co-localization of Olig2 and SC101 (<b>E</b>). (<b>F–J</b>) Some human nuclei-positive cells, SC101 green (<b>H</b>) also express the mature oligodendrocyte marker APC-CC1, red (<b>G</b>), DAPI counterstain, blue (<b>F</b>) Arrows indicate a double-labeled cell. Merged confocal image reveals some hCNS-SCns express APC-CC1 expression 16 weeks after transplantation. (<b>I</b>). Orthogonal view of confocal image revealing co-localization of APC-CC1 and human nuclei marker (<b>J</b>). (<b>K–O</b>) Human cytoplasm-positive cells SC121, green (<b>M</b>) also exhibit ß-tubulin III expression, red (<b>L</b>). DAPI counterstain, blue (<b>K</b>). Arrows indicate a double-labeled cell. Merged confocal image, revealing hCNS-SCns expression of ß-tubulin III (<b>N</b>). Orthogonal view of confocal image showing co-localization of ß-tubulin III and SC121 (<b>O</b>). (<b>P–T</b>) Few human cytoplasm cells SC121, red (<b>Q</b>) also expressed and the astrocyte marker GFAP, green (<b>R</b>). DAPI counterstain, blue (<b>P</b>) Arrowhead indicates a non-astrocytic human cell. Co-localization was rare indicating few hCNS-SCns exhibited astrocytic differentiation 16 weeks after transplantation (<b>R</b>). Orthogonal view of confocal image revealing co-localization of GFAP and SC121 (<b>S</b>). Scale bars = 20 µm and 10 µm in the bottom row.