Expression of miRs-1, 206, 133a and 133b as well as their precursors in the AML 12 cells.

<p>A. AML 12 cells were treated with 10 nm T3 for 1 hour or 24 hours. The expression of miRs-1, 206, 133a and 133b was examined with the Taqman miRNA Assay. U6 was used as an internal control. Five batches of cultured cells were considered as 5 biological replicates. B. The expression of the precursors of miRs-1, 206, 133a and 133b was examined in AML 12 cell treated with 10 nm T3 for 24 hours with RT-PCR. <i>Hprt</i> was used as internal control. Three batches of cultured cells were used as 3 biological replicates. Data are presented as mean ± SE. A two-tailed Student's t-test was used to calculate significance. * indicates p<0.05.