Thymoquinone induces cell death in human glioblastoma cells.

<p>(<b>A</b>) Cell viability of the various cell types after 24 hour treatment with TQ at the different concentrations is shown. The percentage cell viability was normalised against the DMSO controls (0 µM) for each cell type. Mean and standard deviations of the three independent experiments are shown. (<b>B</b>) Cell cycle profiles of the IMR 90, hTERT-BJ1 and glioblastoma cells without (0 µM) or with TQ (50 µM) treatment as measured by propidium iodide staining. (<b>C</b>) Changes in level of cell cycle regulatory proteins in the cell lines tested. Whole cells were lysed and equal amounts of proteins were separated using 4–20% SDS-PAGE, transferred to PVDF membrane and immunoreacted with antibodies against p53 and p21. β-actin was used as loading control.