LAIR-2/Fc inhibits adhesion of platelets to collagen under flow conditions.

<p><i>Panel</i> A: Collagen type III-coated coverslips were perfused with whole blood in the absence or presence of 100 µg/ml soluble LAIR-1/Fc, LAIR-2/Fc or SIRL-1/Fc. Representative pictures are shown. Perfusion was performed at a shear rate of 300 s⁻¹ (upper panels) or 1500 s⁻¹ (lower panels). Percentages below figures indicate percentage surface-coverage for each individual photo. <i>Panels</i> B & C: Quantitative representation of surface coverage of collagen type III-coated coverslips that were perfused in the absence or presence of 100 µg/ml soluble LAIR-1/Fc, LAIR-2/Fc or SIRL-1/Fc at 300 s⁻¹ (<i>panel</i> B) or 1500 s⁻¹ (<i>panel</i> C). Data represent mean±SD of three independent perfusions. <i>Panel</i> D: Dose dependent inhibition of surface platelet coverage in the presence of LAIR-2/Fc at shear rates of 300 s⁻¹ (open symbols) or 1500 s⁻¹ (closed symbols). *: <i>p</i><0.005.