Effect of different stimuli on miR-525-5p expression in human monocytes.

<p>Monocytes from three different donors were used to analyze the effect of LPS, lipoprotein, PMA and CoCl₂ on miR-525-5p expression. (A) Monocytes RU were stimulated with 0.05 µg/ml of LPS from <i>E coli</i> 055:B5; 0.05 µg/ml lipoprotein (LP: Pam2CSK4); 5 nM PMA or 0.05 µg/ml of LPS from <i>E coli</i> 026:B6 for 1 h or 9 h. Relative gene expression in stimulated cells were compared with untreated cells (UNT), which are set to 1. (B, C) Monocyte NE and MA were stimulated with 0.05 µg/ml LPS (from <i>Escherichia coli</i> 055:B), 5 nM PMA and 20 µg/ml CoCl₂ for 1 h and 9 h. UNT was set to 1.