Enhanced infiltration of inflammatory infiltrate contributed to control of acute tissue parasite burden in TcVac2-immunized mice.

<p>Mice were vaccinated with TcVac2 or given vector or cytokines (cyt) alone, and challenged with <i>T. cruzi</i>. (<b>A</b>) Shown are H&E staining (blue-nuclear and pink-muscle/cytoplasm/keratin) of heart tissue and skeletal muscle (Sk Ms) sections at day 30 pi (magnification: 20X). Arrows mark parasite nests in panels b & d. (<b>B&C</b>) Total DNA from Heart (Hrt), Skeletal Muscle (Sk Ms), Spleen (Spl), Liver (Liv) and Kidney (Kid) was isolated and used as a template for the amplification of <i>T. cruzi</i> 18SrDNA sequence by traditional PCR (<b>B</b>) or real time PCR (<b>C</b>). Standard deviation was <12% for the data presented in the <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0000797#pntd-0000797-g005" target="_blank">Figure 5C</a> (^##<i>p</i><0.01, ^###<i>p</i><0.001).