NeuN immunoreactivity in tissue sections.

<p>NeuN positive nuclei in spinal cord at 2-, 24-hours and 4 weeks after injury. At 2 hours (2 h), a small number of neurons were visible at the centre of the injury site located mainly in the dorsal horns. By 24 hours (24 h) there had been further loss of neurons up to 2 mm either side of the centre of the injury site. NeuN profiles at 4 weeks (4w) were similar to that at 24 hours indicating that the loss of grey matter neurons occurs within the first 24 hours (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012021#pone-0012021-g006" target="_blank">Fig. 6</a>).