EAE is reduced by a challenge to the footpad with MOG_35–55 peptide.

<p>EAE was assessed fourteen days after C57BL/6 mice were immunized with MOG peptide_{35<b>–</b>55}, CFA and PTX or immunized and a footpad challenged with intradermal MOG peptide_{35<b>–</b>55} or IRBP peptide_{161<b>–</b>180} 7 days P.I. Following immunization, animals were kept under observation to score the disease. The study was done in a blinded fashion. The EAE scale was as follows: 0 = normal, 1 = limp tail, 2 = paraparesis with a clumsy gait, 3 = hind limb paralysis, 4 = quadriplegia, 5 = death. Data represents the mean score +/− S.E.M. of five mice/point. The experiment was done twice. DTH: delayed type hypersensitivity, chl: challenge