Effects of estradiol (E2) on <i>hpg</i> AVPV and ARC kisspeptin immunostaining patterns.

<p><b>A</b>) Representative images of AVPV kisspeptin immunostaining patterns from gonad intact or ovariectomized (OVX) 60 day old WT and <i>hpg</i> females treated with sham or E2-containing Silastic capsules for 7 days. Scale bar = 100 µm. <b>B</b>) AVPV kisspeptin-immunoreactive neurons from each group in (A) were counted and compared. Values are mean ± SEM. Different letters denote significant differences (ANOVA, Neuman-Keuls post hoc), P<0.05. <b>C)</b> Representative images of kisspeptin immunostaining patterns in the ARC of intact diestrus or OVX female WT or <i>hpg</i> mice, treated with sham or E2-containing Silastic capsules for 7 days. Scale bar = 200 µm.