Experimental Flow Chart for Screening RF-Induced Ca²⁺ Effects.

<p>The experimental work plan for Ca²⁺ experiments consisted of a Screening Phase (1) and Provocation Phase (2). A screening phase was used to expose all cell types tested to a 2 W/kg 900 MHz GSM field for 30 minutes. If significant changes in Ca²⁺ were observed in the exposure period, additional experiments would be performed at lower SARs to determine the threshold for the effect. If, however, no significant effects were observed in the screening phase, provocation experiments would be undertaken where the Ca²⁺ homeostasis would be deliberately perturbed. For these experiments, the human endothelial cell line (EA.hy926), was chosen and tested at a number of lower SARs, using both continuous wave and GSM modulation conditions.