Differential interaction of TPR-proteins with PR heterocomplexes.

<p>HEK-293 cells were transfected as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011717#pone-0011717-g007" target="_blank">figure 7</a>, except that HA-MR was expressed instead of HA-GR. Cells were processed and protein interactions were analyzed also as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011717#pone-0011717-g007" target="_blank">figure 7</a>. In A, binding of TPR-proteins is presented relative to the mean of the normalized FLAG-eluate signals of CHIP, FKBP52, PP5 and TPR2. Quantification represents means of three independent experiments (two for FKBP51) +S.E.M. In B, binding is normalized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011717#pone-0011717-g007" target="_blank">figure 7</a>. C, FLAG- and HA-immunoblot signals of the cell extracts, demonstrating expression of TPR proteins and PR. Quantifications represent means of three independent experiments +S.E.M.