CD4⁺ T cells maintain contact with CD11c-EYFP⁺ cells in both acute and chronic granulomas.

<p>A, Quantitative analysis of CD4⁺ T cell contact with CD11c-EYFP⁺ cells in granulomas from three- and ten-week liver sections. All CD4⁺ T cells per granuloma section were determined to be in contact (green bars), or not in contact (white bars) with CD11c-EYFP⁺ cells. For each time point, liver sections from 3 independent mice were used, and 10–15 lesions per section were analyzed. Data are represented as mean +/− SEM, <i>P<0.0001</i>. B, Fluorescent images demonstrating CD11c-EYFP contact with CD4⁺ cells. Granulomas are outlined with white dashed lines at 1000× magnification and colors shown depict CD11c-EYFP (green cells), anti-CD4 Alexa 647 (red cells), and DAPI nuclear stain (blue).