Determination of the presence of infectious HIV-1 in CVL from HIV(+) women.

<p>HIV(+) CVL from 32 patients were diluted 1∶4 and added directly to TZM-bl cells. The assay was terminated 48h post-infection and HIV-1 infection was quantified by measuring luciferase reporter gene activity using a luminometer and expressed as relative light units (RLU). Each data point in graph represents one individual patient. In the Control (media only) column, each point represents replicate wells. When comparing Controls with the CVL samples (TZM-bl cells incubated with CVL), 3 out of 32 showed RLU levels at least 2-fold above background indicating that these 3 CVL contained infectious HIV-1 which trans-activated the LTR-driven reporter gene.