<i>asc1Δ</i> cells display aberrant cell-wall morphologies upon exposure to hypotonic conditions.

<p>Microscopic observations of wild-type and <i>asc1Δ</i> cells either under sustained growth in rich media (YPD) containing 1M NaCl (A, D), or following one hour exposure to hypotonic shock conditions (shift from 1 M to 0 M NaCl containing YPD media)(B, E), or in hypotonic conditions in the presence of 0.1 mg/ml Cycloheximide (CHX) (C, F). Arrows point to cell-wall deformations sites. Inset in E is a higher magnification of deformed cells.