Post transplant administration of AMD3100 selectively enhances reconstitution of all donor-derived hematological lineages of cells.

<p>KTLS cells from C57BL/Ka CD45.1 Thy1.1 mice were injected via tail vein into lethally irradiated C57BL/6 CD45.2 Thy1.2 mice (250 KTLS cells per mouse). Beginning 2 days after transplant (day +2), the mice were injected subcutaneously with PBS control or AMD3100 at 5 mg/kg body weight every Monday, Wednesday, and Friday until day +56. Hematological recovery and cell subset analyses were determined at the time points indicated, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011316#s4" target="_blank">Methods</a>. The graphs are representative of 3 separate sets of experiments with 10 mice in each group in each experiment. A: Blood nucleated cell counts (/µl) (normal range of mouse white cell counts: 1.8–10.7×10³/µl). B: Donor-derived CD45.1⁺ cell counts (/µl). C: Blood recipient origin CD45.2⁺ cell counts (/µl). D: Blood CD3⁺ T cell counts (/µl). E: Blood B220⁺ B cell counts (/µl). F: Hemoglobin concentration (g/dl) (normal range of mouse hemoglobin level: 11–15.1 g/dl). G: Blood platelet counts (×10⁶/ml) (normal range of mouse platelet count: 592–2972×10⁶/ml). *: p<0.05