Post transplant administration of AMD3100 enhances donor cell marrow engraftment.

<p>The mice as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011316#pone-0011316-g001" target="_blank"><b>Fig. 1</b></a> were sacrificed at Day +65 post transplantation, and 2 femurs and 2 tibias were harvested and processed and analyzed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011316#s4" target="_blank">Methods</a>. A: CFU-GM assay. CFU-GM colonies were measured in individual mice using MethoCult® GF 3434 mouse colony-forming cell assay. B: Histology. Bone marrow from individual mouse was examined by microscopy (4X: top panel and 100X: bottom panel) after decalcification and H/E staining. Trabecular bony structure, osteoblasts, hematopoietic cells, sinusoid vessels and acellular areas filled with lipid droplets were indicated. Normal mice did not receive transplant or AMD3100 injection. C: Total nucleated cell number from 1 femur and 1 tibia. D: Donor-derived CD45.1⁺ KLS stem cell number from 1 femur and 1 tibia of individual mouse. E: Recipient-origin CD45.2⁺ KLS stem cell number from 1 femur and 1 tibia of individual mouse. *: p<0.05.