Characteristics of peptide:MHC cellular microarrays.

<p>(A) A part of a freshly fabricated microarray image with magnification of 15.75× (left panel), 100× (middle panel) and 200× (right panel). (B) A raw fluorescence image of a 4-supergrid 8×12 microarray probed with Cy3-conjugated anti-Rat-IgG. The absolute fluorescence of all 96 spots for the low-right supergrid was quantified and analyzed for the variance. (C) A microarray loaded with 6×10⁶ primary human CD4 T cells. (Left panel, 25× magnification) image taken immediately after 6 hours of incubation; (middle panel, 200× magnification) image taken after aspirating culture medium; (right panel) image taken after washing the slide with 1xPBS for three times. The numbers at the low-right corners of middle and right panels indicate cell counts. (D) The box/whisker plots represent cell counts of 20 randomly selected spots before (loose attached) and after (tightly attached) washing procedure.