Sphingolipid levels in the livers of DIO mice after treatment with Genz-112638.

<p>C57BL/6 mice were fed a normal diet (Lean) or a HFD (DIO) for 8 weeks. The DIO mice were then treated with Genz-112638 or placebo (water) by twice daily oral gavage (120 mg/kg/day) for 22 weeks, while remaining on the HFD. Sphingolipid levels were determined by high performance liquid chromatography – tandem mass spectrometry (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011239#s4" target="_blank">Materials and Methods</a>). Data shown as mean ± SEM (n = 6–9 mice per group). *<i>P</i><0.05, **<i>P</i><0.01 Genz-112638 DIO vs. Water DIO.