The functional profile of CD4+ and CD8+ T cells is increased in TB cases but reduced post-treatment.

<p>PBMC were stimulated overnight using ESAT-6/CFP-10 and CD4+ and CD8+ cells were analysed by flow cytometry for different combinations of IFN-γ, IL-2, TNF-α and CD107a. Responses are grouped and colour coded according to the number of factors: White = 1 function, black = any 2 factors, blue = any 3 factors and red = all 4 factors. The pie charts summarise the results shown in the dot plots indicating the combination of cytokines as a percent of total responding cells for CD4+ (A) and CD8+ (B). Significant differences are indicated and were determined using a Kruskal-Wallis ANOVA followed by Dunn's post-test comparison. Pie chart segments were analysed using a permutation test and p-values indicated.