Multiplex genome-wide exon amplification based on MYBPC3 gene exons 7 and 8.

<p>The human genomic DNA was PCR-amplified under standard conditions at 58°C with primers designed from the donor (5′) splice signal sequence (from exon 7) and the acceptor (3′) splice signal sequence (from exon 8) of the MYBPC3 gene. It was also amplified by the same FR primer pairs with decreasing number of fixed bases and increasing number of random bases (Ns) as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011138#pone-0011138-t001" target="_blank">Table 1</a>. The expected fragment (438 bases for the combined exon 7, intron 7 and exon 8) is present in all the lanes, and the number of fragments amplified increased with increasing Ns in the FR primers. M1 & M2 are marker lanes. Lane CS shows the computer simulated exon fingerprint obtained with the same primers used for lane 5, with four bases removed from the 5′ end of the forward primer and three bases removed from the 5′ end of the reverse primer (see text).