Production of cytokines in human PBMC/DCs co-cultured with recombinant <i>Lactobacillus</i>.

<p>Human Peripheral Blood Mononuclear Cells (PBMCs) were treated with 100 nM GM-CSF and 10 nM IL-4 during 5 days to derive monocytes into dendritic cells. 10⁶ cells/well were seed in 24 well plates and co-cultured with UV-killed recombinant <i>Lactobacillus</i> expressing OspA (LpA) or the mutant OspA_D17 (LpA_D17) at MOI 10:1 colony-forming units per cell. 100 ng/ml <i>Escherichia coli</i> O111:B4 lipopolysaccharide (LPS) and <i>L. plantarum</i> (Lp) were used as positive and negative control, respectively. After 48 h of stimulation, supernatants were collected and TNFα (A), IL-12 (B), IFNγ (C), IL-6 (D) and IL-10 (E) cytokine production was measured by sandwich ELISA (Quantikine). *<i>p</i><0.001,**<i>p</i><0.02. Results are representative of one of three independent experiments.