Recombinant PorB and evaluation of antibody response against <i>F.tularensis</i> LPS.

<p>(A) The band of rPorB detected by Coomassie gel stain is indicated by the arrow. (B) Serum was collected four weeks after vaccination, just before i.n. challenge. Antibody production was analyzed by ELISA and results are presented as nanograms (ng) of LPS-specific IgG and IgM per milliliter (ml) of serum. Black dots indicate individual mice, while bars represent mean values. Lower antibody concentrations include values between 286 and 436 ng/ml as opposed to those that were not detected (ND) in control groups. Significant differences were calculated by Mann Whitney test and are indicated by *** (<i>P</i><0.001) for LPS+rPorB compared with PBS.