Effects of tetraspanins on CD9P1-induced cell motility on collagen I.

<p>(A) HEK/CD9P-1 cells were transfected with tetraspanins CD9, CD81, CD82, CD151 or CD9×82. Then, cells were plated on collagen I. Time-lapse videomicroscopy was performed at a rate of 1 frame per 15 min for 24h. The results correspond to six independent experiments; standard errors are shown. (B) Time-lapse videomicroscopy experiments were performed with HEK/CD9P-1 cells transfected with CD9 or CD82. The mAbs directed against integrins α1 (FB12) or α2 (Gi9) were added to the media (10 µg/ml). Three independent experiments were performed; standard errors are shown. (*) p<0.05, (**) p<0.01.