3DG-collagen stimulates intracellular ROS in fibroblasts.

<p>Fibroblasts were cultured in 96-well plate coated with either native collagen or 1 mM 3DG-collagen or treated with or without 5 mM AG, 40 mM meglumine, or 100 µg/mL ascorbic acid for 24 h. Treatment of fibroblasts cultured on native collagen with 50 µM H₂O₂ was used as a positive control. Fibroblasts were loaded with DCFH-DA for 30 min and ROS production was measured by absorbance of fluorescent DCF at a wavelength of 480 nm/530 nm. Comparisons are made to collagen treated with PBS unless otherwise indicated. Data are mean±SD (n = 3), *P<0.001.