Germinal center B cells are exclusively found within BALT from LPS+rPorB vaccinated/LVS challenged mice.

<p>(A) Representative lung from naïve negative control; (B) mediastinal lymph node from LPS+rPorB/challenged mouse as an internal positive control; (C) Lung from LPS vaccinated/challenged; (D, E) Lung from LPS+rPorB vaccinated/challenged mouse and (F) lung from LPS+PorB vaccinated/unchallenged mouse. Cells markers are B220 for B cells (red- Alexa 549) and PNA to detect germinal center B cells (green- Alexa 488). Areas of color overlap indicate germinal centers (see arrows). Cell nuclei were stained with 4′-6-Diamino-2-phenylindole (DAPI-blue). Representative pictures were taken with a 20× objective.