Allogeneic mixed-lymphocyte reaction (MLR).

<p>DCs (stimulators, <b>A</b>) from dog peripheral blood monocytes cultured with either Ad.hFlt3L conditioned media (purple dots) or IL-4+GM-CSF (orange dots) were incubated with allogeneic spleen T cells (responders, <b>B</b>) purified by Fluorescence Activated Cell Sorting (FACS) from the spleen of a second dog. Dot plots show the percentage of CD11c⁺-cells (<b>A</b>) and the percentage of CD8⁺ and CD4⁺ T cells collected from dog spleen by FACS (<b>B</b>). SSC: Side Scatter; FSC: Forward Scatter. <b>C</b>, T cell proliferation was determined by BrdU incorporation after 4 days in co-culture. Additional controls included DCs incubated without T cells (broken lines). *p<0.05 vs. DCs no T cells (Randomization test).