Increased IFNα expression by pDC in lymph nodes of HIV-1 patients.

<p>Panel A: flow cytometry plot showing expression of IFNα by lymph node-homed pDC from a representative healthy control and an HIV-1 patient. Histogram plot was obtained by gating onto the live cell population as assessed by forward and side light scatter profile (not shown), and then onto the pDC population as identified by staining with anti-BDCA2 and anti-CD123 antibodies. Top density plot and gray area histogram: healthy control; bottom density plot and black line histogram: HIV-1 patient. Panel B: summary results for expression levels of IFNα by lymph node-homed pDC of healthy controls (black bar; <i>n</i> = 11) and HIV-1 patients (white bar; <i>n</i> = 18). We assessed the Geometric Mean Fluorescence Intensity (GMFI) value with cells from all individuals in the two study groups (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011110#s2" target="_blank">Materials and Methods</a>). Panel shows the Mean and SEM of the GMFI values.