<p>(A) DNA from type A, C, D or E isolates was subjected to PFGE prior to Southern blotting and hybridization with a DIG-labeled, <i>cpe</i>-specific probe. (B) DNA from type A (F5603) or type C (CN2076, CN3748, CN3758, CN3763 and CN3753) isolates was subjected to PFGE prior to Southern blotting and hybridization with a DIG-labeled, <i>cpe</i>-specific probe. The migration of molecular size markers is indicated on the left of the blot.</p
<p>DNA restriction fragments obtained from blood samples of all available members of family A (lanes...
<p>(<b>A)</b> PFGE-<i>Dra</i>I and (<b>B)</b> Southern blot hybridization using the <i>trfA</i>-deri...
<p>Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern hybridization p...
<p>DNA from each isolate was digested with XbaI prior to conventional agarose gel electrophoresis an...
<p>DNA digested with <i>Pst</i>I from <i>cpe</i>-positive and <i>cpe</i>-negative (M-08 and MR2-12) ...
<p>Representative results obtained with this assay are shown for type A isolates known to carry a ch...
Pst I cut genomic DNA was hybridized and blotted as summarized in the Methods section. Sizes of the ...
<p>Southern blotting of PFGE was performed with DIG-labelled probes for <i>netE</i> and <i>cpe</i> g...
<p>An overlapping PCR assay specific for amplification of the type D isolate CN4003 <i>cpe</i> locus...
<p>An overlapping PCR assay specific for amplification of the type C isolate CN2078 <i>cpe</i> locus...
<p><b><i>M. avium</i></b><b> and </b><b><i>M. kansasii</i></b><b> colonies.</b> (A) PFGE with undige...
<p>(A and C) undigested PFGE of isolated DNA (B) hybridization using a <i>boNT</i>/A probe (D) hybri...
<p>A) Probe design. A 566 bp probe was used, recognizing a region of eGFP gene. Dashed box correspon...
<p>A: Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern blot hybridi...
<div><p>(A) A Southern blot using a 1.1 kb probe overlying <i>CFH</i> exon 21 and <i>CFHL1</i> exon ...
<p>DNA restriction fragments obtained from blood samples of all available members of family A (lanes...
<p>(<b>A)</b> PFGE-<i>Dra</i>I and (<b>B)</b> Southern blot hybridization using the <i>trfA</i>-deri...
<p>Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern hybridization p...
<p>DNA from each isolate was digested with XbaI prior to conventional agarose gel electrophoresis an...
<p>DNA digested with <i>Pst</i>I from <i>cpe</i>-positive and <i>cpe</i>-negative (M-08 and MR2-12) ...
<p>Representative results obtained with this assay are shown for type A isolates known to carry a ch...
Pst I cut genomic DNA was hybridized and blotted as summarized in the Methods section. Sizes of the ...
<p>Southern blotting of PFGE was performed with DIG-labelled probes for <i>netE</i> and <i>cpe</i> g...
<p>An overlapping PCR assay specific for amplification of the type D isolate CN4003 <i>cpe</i> locus...
<p>An overlapping PCR assay specific for amplification of the type C isolate CN2078 <i>cpe</i> locus...
<p><b><i>M. avium</i></b><b> and </b><b><i>M. kansasii</i></b><b> colonies.</b> (A) PFGE with undige...
<p>(A and C) undigested PFGE of isolated DNA (B) hybridization using a <i>boNT</i>/A probe (D) hybri...
<p>A) Probe design. A 566 bp probe was used, recognizing a region of eGFP gene. Dashed box correspon...
<p>A: Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern blot hybridi...
<div><p>(A) A Southern blot using a 1.1 kb probe overlying <i>CFH</i> exon 21 and <i>CFHL1</i> exon ...
<p>DNA restriction fragments obtained from blood samples of all available members of family A (lanes...
<p>(<b>A)</b> PFGE-<i>Dra</i>I and (<b>B)</b> Southern blot hybridization using the <i>trfA</i>-deri...
<p>Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern hybridization p...