Analysis of <i>cpe</i> locus diversity in type C and D isolates using a multiplex PCR subtyping assay for <i>cpe</i> loci commonly found in type A isolates.

<p>Representative results obtained with this assay are shown for type A isolates known to carry a chromosomal <i>cpe</i> gene (SM101), a plasmid <i>cpe</i> gene with an associated IS<i>1470</i>-like sequence (F4969), or a plasmid <i>cpe</i> gene with an associated IS<i>1151</i> sequence (F5603). Also shown are representative results for this assay using culture lysates from <i>cpe</i>-positive type C isolates (CN2078, NCTC5388), <i>cpe</i>-positive type D isolates (CN4003) and type E isolates carrying silent <i>cpe</i> sequences (853 and NCIB10748). The migration of molecular size markers is indicated on the left of the blot.