Bryostatin synergizes Tat- and Nef-mediated LTR-transactivation.

<p>SVGA-LTR-GFP reporter cells were transfected with 2 ng Tat-expression vector (suboptimal dose) and increasing concentrations of Nef-expression vector. (<b>A</b>) Quantification of GFP-positive cells. (<b>B</b>) SVGA-LTR GFP cells were transfected with 2 ng Tat-expression vector and 100 ng Nef-expression vector in the presence or absence of bryostatin (25 ng/ml) followed by GFP quantification using flowcytometry. (<b>C</b>) Mean fluorescence intensity (MFI) for the same experiment (<b>B</b>). (n = 3).