Cell cycle arrest by siRNA.

<p><b>A)</b> Cells were transfected without siRNA, with scramble siRNA (scramb) or with siRNA against cyclin D1 or cyclin-kinase (CDK4). Forty-eight hours post-transfection cells were infected with VSV-wt at an MOI of 0.1 for 24 hours. Results show the average of at least three independent experiments. <b>B)</b> Mock-infected lysates from PH5CH8 and Huh-7 cells of the experiment describe above are shown for the expression of cyclin D1 and CDK4. <b>C)</b> FACS analysis of cell cycle arrest in Huh-7 cells upon treatment with siRNA targeting CDK4 and cyclin D1 or control siRNA (SCR). Experiments were conducted at least three times and triplicate values of one experiment are shown as representative (p<0.001).