Effects of 4-1BB ligation in spleen cells of NP- and P-mice stimulated with Ag85B protein on expression of activation markers on CD4⁺ T cells.

<p>Unfractionated spleen cells of P- or NP-mice were cultured with Ag85B protein (5 µg/ml) in the presence of IgG2a control Ab or agonist anti-4-1BB mAb (5 µg/ml). After 6 days of culture cells were labeled with the indicated mAbs. Cell surface molecule expression was analyzed by flow cytometry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011019#s4" target="_blank">Materials and Methods</a>. The results are presented as mean ± SEM of the percentage of CD4⁺ T cells staining for the indicated markers. Pooled data of 3 independent experiments are shown.</p><p>#, § statistical significance (p<0.05 or <0.01) for differences between groups determined by ANOVA and Bonferroni-type multiple t-test (# Ag85B/control IgG vs unstimulated; § Ag85B/control IgG vs Ag85B/anti-4-1BB mAb).