IFN-treatment increases steady-state levels of ERα mRNA and protein in mouse breast cancer cell line WT276.

<p>(A) Total protein extracts were prepared from control (lane 1), increasing concentrations (1,000 or 2000 u/ml) of IFN-α (lanes 2 and 3, respectively) or IFN-γ (5 or 10 ng/ml; lanes 4 and 5, respectively) treated WT276 cells. As a negative control, we also included extracts from AKR-2B cells. The extracts were analyzed by immunoblotting using the antibodies specific to the indicated proteins. Fold change in ERα protein levels is indicated below the Figure. (B) Total RNA was isolated from control (lane 1), increasing concentrations of IFN-α (lanes 2 and 3) or IFN-γ (lanes 4 and 5) treated WT276 cells. As a positive control, we also included RNA from splenic cells. The total RNA was analyzed for steady-state levels of <i>Esr1</i> mRNA by semi-quantitative RT-PCR. Fold change in ERα mRNA levels is indicated below the Figure. (C) Total RNA was isolated from control (column 1), increasing concentrations of IFN-α (columns 2 and 3) or IFN-γ (columns 4 and 5) treated WT276 cells. The total RNA was analyzed for steady-state levels of <i>Esr1</i> mRNA by quantitative real-time PCR. Results are mean values of triplicate experiments and error bars represent standard deviation (^*<i>p</i><0.05).