Spleen cells from male and female infected mice secrete different levels of cytokines after paracoccin stimulus.

<p>Spleen cells of mice of both sexes (n = 6) infected with <i>P.brasiliensis</i> yeast were collected after 7 and 30 days post infection and cultured for 48 hours under stimulation with paracoccin (0.7 µg/ml), LPS (1 µg/ml) or only medium. The concentrations of IL-12p40, TNF-α, IFN-γ and IL-10 in supernatants were measured by ELISA. Each bar represents the mean ± SD of duplicates and is representative of four experiments made in duplicate. * p<0.05 significant female versus male of the same group. All cytokine data were normalized against those of the non-infected male and female mice.