Prolonged G2/M delay upon brief exposure of H3-K56R/A/Q-S57E mutants to MMS in G1.

<p>The indicated yeast strains were arrested in G1 with mating pheromone and divided into two samples that were exposed (bottom) or not (top) to 0.1% MMS for 20 minutes prior to release from a G1 arrest into complete growth medium. Cell cycle progression was measured as a function of cellular DNA content in samples collected at the indicated time.