NO production and viability of Pb yeasts after phagocytosis by macrophages from male and female mice.

<p>Macrophages from male and female mice were incubated with paracoccin (PCN), <i>P. brasiliensis</i> yeasts (Pb), PCN plus Pb, PCN (pre-treated with GlcNac) plus Pb or only medium and nitrite levels were detected by the Griess method (4A). For the evaluation of viability of Pb cells macrophages were stimulated with PCN, PCN (pre-treated with GlcNac) or only medium and challenged with live Pb. The determination of the number of viable fungi was made by CFU counts (4B). The results represent the mean±SD from a representative experiment of three assays. * p<0.05 significant when compared with male of the same group.