High glucose, palmitate or cytokines do not affect human islet insulin mRNA levels.

<p>Human islets were cultured and analyzed by real-time RT-PCR as given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010843#pone-0010843-g001" target="_blank">Figure 1</a>. Primers specific for insulin mRNA were used. Results are means ± SEM for 5 independent experiments.